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Laser scanning microscope system Zeiss LSM 510 NLO Light Sources
CW multiline Argon ion laser (458, 477, 488, 514 nm), CW He-Ne laser (543 nm), Femtosecond broadband laser Spectra-Physics Tsunami (tuned 700-1000 nm, average pulse duration is 100 fs) for multi-photon excitation. Zeiss LSM 510 NLO
Techniques available in transmitted and reflected light Brightfield and darkfield microscopy; Phase contrast, differential interference contrast, modulation contrast; Polarization microscopy; Fluorescence microscopy with wide-field excitation by mercury lamp lines may be used for thin samples. Microspectroscopy and fluorescence lifetime imaging (FLIM) based on single photon counting.
Technical charcteristics Image resolution 2048 x 2048 pixels Types of optical response Fluorescence, harmonic generation, reflected and transmitted signal, Raman lines, etc.).
Applications Conformation analysis of biological objects Material charactarization, nondestructive testing of various materials at micro- and nano-level. Time-correlated single photon counting module Becker & Hickl SPC-140 Technical Characteristics PCI time correlated single photon counting module; electrical time resolution down to 8 ps FWHM / 5 ps rms; up to 65536 (256 x 256) decay curves in memory; count rate up to 3 MHz; measurement times down to 1 ms; multi detector capability; fast object scanning / lifetime imaging modes
Application Fluorescence Kinetics, Fluorescence Lifetime Imaging (FLIM), Laser Scanning Microscopy, Two Photon Microscopy, Fluorescence Resonance Energy Transfer (FRET)
Scanning Near-Field Optical Microscopy Scanner Sample position - horizontal, X,Y scan range - 20 µm x 20 µm, Z scan range - 5 µm, Initial Z approach - slip-and-stick motion upward or downward, automatically controlled. Coarse slip-and-stick motion in x, y plane - step 1 µm, the full range - 10 mm.
Probes Standard optical fiber probes for SNOM glued onto a tuning fork (standard watch quartz resonator for 32 kHz)
Controller electronics Electronic unit specially designed to work with the 32 kHz resonance frequency tuning fork with an attached probe. The performance of such a detector is really close to the theoretical noise and resolution limit.
Frequency range - 31 - 33 kHz, Frequency accuracy - < 0.01 Hz, Frequency stability f/f -d < 10-8, Force sensitivity - 1 pN/ÖHz, Response time - 2 ms. Photon detection Electronics provides the possibility to count the photons in a time gating mode with an external synchronization (the shortest time interval available is 100 ns). Both direct output of PMT (negative single-photon pulses with an amplitude of 10 mV and duration of a few ns) or TTL - pulses from other counters can be used.
Light Sources CW He-Ne laser (632 nm), CW He-Cd laser (441 nm, 325nm), CW YAG laser (second harmonic - 532 nm)
Applications Characterization of metal and semiconductor nanoparticles, study of physical characteristics of biological objects, nondestructive testing of various materials at micro- and nano-level, nanolithography, nanophotonics, metal plasmon waveguides
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